Fit GLM with bootstrap resampling

cytoglm(
  df_samples_subset,
  protein_names,
  condition,
  group = "donor",
  covariate_names = NULL,
  cell_n_min = Inf,
  cell_n_subsample = 0,
  num_boot = 100,
  num_cores = 1
)

Arguments

df_samples_subset	Data frame or tibble with proteins counts, cell condition, and group information
protein_names	A vector of column names of protein to use in the analysis
condition	The column name of the condition variable
group	The column name of the group variable
covariate_names	The column names of covariates
cell_n_min	Remove samples that are below this cell counts threshold
cell_n_subsample	Subsample samples to have this maximum cell count
num_boot	Number of bootstrap samples
num_cores	Number of computing cores

Value

A list of class cytoglm containing

tb_coef

coefficent table

df_samples_subset

possibly subsampled df_samples_subset table

protein_names

input protein names

condition

input condition variable

group

input group names

covariate_names

input covariates

cell_n_min

input cell_n_min

cell_n_subsample

input cell_n_subsample

unpaired

true if unpaired samples were provided as input

num_boot

input num_boot

num_cores

input num_cores

formula_str

formula use in the regression model

Examples

set.seed(23)
df <- generate_data()
protein_names <- names(df)[3:12]
df <- dplyr::mutate_at(df, protein_names, function(x) asinh(x/5))
glm_fit <- CytoGLMM::cytoglm(df,
                             protein_names = protein_names,
                             condition = "condition",
                             group = "donor",
                             num_boot = 10) # in practice >=1000
glm_fit
#> 
#> #######################
#> ## paired analysis ####
#> #######################
#> 
#> number of bootstrap samples: 10 
#> 
#> number of cells per group and condition:   
#>     control treatment
#>   1    1000      1000
#>   2    1000      1000
#>   3    1000      1000
#>   4    1000      1000
#>   5    1000      1000
#>   6    1000      1000
#>   7    1000      1000
#>   8    1000      1000
#> 
#> proteins included in the analysis:
#>  m01 m02 m03 m04 m05 m06 m07 m08 m09 m10 
#> 
#> condition compared: condition 
#> grouping variable: donor